Human Anti-Human CD27 Recombinant Antibody (TAB-H74) (CAT#: TAB-H74)
Recombinant human antibody to Human CD27. This product is designed to enhance the body's natural immune response by directly activating T cells that can specifically recognize and kill cancer cells.
Specific Inquiry
SDS-PAGE
Figure 1 Human CD27 Recombinant Antibody (TAB-H74) in SDS-PAGE
SDS-PAGE analysis of TAB-H74 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
SEC-HPLC
Figure 2 Human CD27 Recombinant Antibody (TAB-H74) in HPLC
The purity of TAB-H74 was greater than 95% as determined by SEC-HPLC.
Specifications
- Host Species
- human
- Derivation
- human
- Type
- IgG1 - kappa
- Specificity
- CD27 (TNFRSF7, tumor necrosis factor receptor superfamily member 7) [Homo sapiens]
- Species Reactivity
- Human
- Applications
- ELISA, IP, FC, FuncS, Neut, IF, ICC
- MW
- 146.0 kDa
- Related Disease
- Solid tumors
Product Property
- Purity
- >95%, by SDS-PAGE with silver staining, under reducing conditions.
Applications
- Application Notes
- The CD27 antibody has been reported in applications of ELISA, FC, S-ELISA, Stim, CFSE, Inhib, Activ.
ELISA: Purified T cells (1 × 105 T cells/well) were added to wells of a 96 well plate or 1.5 × 106 cells/well of a 24 well plate) and stimulated with Abs for different durations from 24 to 72 h at 37 °C in a humidified CO2 incubator. Cells were cultured in RPMI 1640 basal medium supplemented with 10 % heat-inactivated fetal bovine serum, L-Glutamine, and penicillin/streptomycin. Cell-free culture supernatants were obtained by centrifugation and frozen at −20 °C for cytokine and biomarker analysis.
Target
Related Resources
Please refer to Varlilumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Varlilumab.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CD27"
Select a product category from the dropdown menu below to view related products.
Please select product type
Mouse Antibody Products
Chimeric Antibody Products
Humanized Antibody Products
Neutralizing Antibody Products
Rabbit Monoclonal Antibody Products
Agonistic Antibody Products
IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
ADCC Enhanced Antibody Products
Antibody Magnetic Beads
Hexamer Antibody Products
Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-063LC | Mouse Anti-CD27 Recombinant Antibody (TAB-063LC) | ELISA, Stim, FuncS | Mouse IgG1, κ |
| TAB-013LC-F(E) | Rat Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-013LC-F(E)) | ELISA | Rat Fab |
| TAB-014LC-F(E) | Rat Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-014LC-F(E)) | ELISA | Rat Fab |
| TAB-015LC-F(E) | Rat Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-015LC-F(E)) | ELISA | Rat Fab |
| TAB-016LC-F(E) | Rat Anti-CD27 Recombinant Antibody; Fab Fragment (TAB-016LC-F(E)) | ELISA | Rat Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for TAB-H74. Click the button above to contact us or submit your feedback about this product.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-H74, RRID: AB_3112045)
Copy citation
Popular products with customers
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: Neut, ELISA, IF, IP, FuncS, FC
Application: EM, ELISA, ICC, IHC-Fr, IHC-P, WB
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
